Rabbit Calnexin Antibody

SKU: orb1524361

Description

Calnexin Antibody

Research Area

Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: Calnexin
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

calnexin; CNX; epididymis secretory sperm binding protein; IP90; major histocompatibility complex class I antigen-binding protein p88; P90

Detection of human Calnexin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Calnexin antibody orb1524361 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Calnexin was also immunoprecipitated by rabbit anti-Calnexin antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Detection of human Calnexin by immunohistochemistry. Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-Calnexin Cat. No. orb1524361 used at a dilution of 1: 1, 000 (1µg/ml) (left) , Cat. No. orb1524359 used at a dilution of 1: 1, 000 (1µg/ml) (center) and Cat. No. orb1524357 used at a dilution of 1: 1, 000 (1µg/ml) (right). Detection: DAB

Quick Database Links

Gene Symbol

Calnexin

UniProt

P27824

RefSeq (Protein):NP_001737.1