Rabbit c-Myb Antibody

SKU: orb1523550

Description

c-Myb Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. Use rabbit anti-c-Myb antibody A304-138A.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: c-Myb
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cmyb; c-myb; c-myb_CDS; efg; oncogene AMV; proto-oncogene c-Myb; transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog

Detection of human c-Myb by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT-4 cells. Antibodies: Affinity purified rabbit anti-c-Myb antibody orb1523550 used for IP at 6 µg/mg lysate.

Detection of mouse c-Myb by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-c-Myb antibody (Cat. No. orb1523550) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human c-Myb by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and salivary gland (right). Antibody: Affinity purified rabbit anti-c-Myb antibody (Cat. No. orb1523550) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

c-Myb

UniProt

P10242

RefSeq (Protein):NP_005366.2