Rabbit anti-BTBD12 Antibody

• Catalog Number: orb1526924
• Category: Antibodies
• Description: BTBD12 Antibody
• Target: BTBD12
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 1784 and C-terminus
• UniProt ID: Q8IY92
• Tested applications: IHC, IP
• Dilution range: WB - Not recommended. orb1526924 has not performed satisfactorily when used for WB of BTBD12 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BTBD12.; IP - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: BTB/POZ domain-containing protein 12; BTBD12; FANC
• Note: For research use only
• NCBI: NP_115820.2

Detection of human BTBD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTBD12 antibody orb1526924 used for IP at 10 µg/mg lysate.

Detection of human BTBD12 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BTBD12 (Cat. No. orb1526924) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB