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Catalog Number | orb1529587 |
---|---|
Category | Antibodies |
Description | BRG1/SMARCA4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 75 and 125 |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | BRG1/SMARCA4 |
UniProt ID | P51532 |
NCBI | NP_003063.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | SWI/SNF-related matrix-associated actin-dependent Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human BRG1/SMARCA4 by western blot.Samples: Whole cell lysate from Jurkat, K-562, HEK293T, MCF-7, and HeLa cells. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody at 0.04 µg/ml.
Detection of mouse BRG1/SMARCA4 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody at 0.04 µg/ml.
Detection of mouse BRG1/SMARCA4 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 used at a dilution of 1:1, 000 (0.2µg/ml).
Detection of human BRG1/SMARCA4 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-BRG1/SMARCA4 antibody.
IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |