Rabbit anti-BORG4 Antibody

SKU: orb1526728

Description

BORG4 Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution range: WB - Not recommended. orb1526728 has not performed satisfactorily when used for WB of BORG4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BORG4.; IP - 5 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 250 and 300
• Target: BORG4
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

cdc42 effector protein 4; KAIA1777; CEP4; BORG4; binder of Rho GTPases 4; CDC42 effector protein (Rho GTPase binding) 4

Detection of human BORG4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BORG4 antibody orb1526728 used for IP at 10 µg/mg lysate.

Detection of human BORG4 by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-BORG4 (Cat. No. orb1526728) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

BORG4

UniProt

Q9H3Q1

RefSeq (Protein):NP_036253.2