Rabbit Bin1 Antibody

SKU: orb1526102

Description

Bin1 Antibody

Research Area

Cancer Biology, Cardiovascular Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 543 and C-terminus
• Target: Bin1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMPH2; amphiphysin II; amphiphysin-like protein; AMPHL; box dependant MYC interacting protein 1; box-dependent myc-interacting protein 1; Bridging integrator 1; CNM2; myc box-dependent-interacting protein 1; SH3P9

Detection of human Bin1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody orb1526102 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Bin1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody orb1526102 used for IP at 6 µg per reaction.

Detection of human Bin1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Bin1 (Cat. No. orb1526102) used at a dilution of 1: 1, 000 (1µg/ml) Detection: DAB

Quick Database Links

Gene Symbol

Bin1

UniProt

O00499

RefSeq (Protein):NP_647593.1