Rabbit ASH2 Antibody

SKU: orb1530118

Description

ASH2 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ChIP, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:20,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ChIP - 1 - 5 µg. Previous lots of this antibody have performed in this application.
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 578 and C-terminus
• Target: ASH2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASH2; ash2 (absent, small, or homeotic)-like; ASH2L1; ASH2L2; ASH2-like protein; Bre2; set1/Ash2 histone methyltransferase complex subunit ASH2

Detection of human ASH2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ASH2 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human ASH2 by WB and IP.Samples: A. HEK293T cells. B. one 10cm plate of normal 293T cells. Antibodies: Affinity purified rabbit anti-ASH2 antibody orb1530118 used at 1 µg/ml for WB (A and B) and at 5 µg/plate for IP.

ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA.

Quick Database Links

Gene Symbol

ASH2

UniProt

Q9UBL3

RefSeq (Protein):NP_004665.1