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Catalog Number | orb1529934 |
---|---|
Category | Antibodies |
Description | AF9 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 275 and 325 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:5,000 - 1:15,000; IP - 1 - 4 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | AF9 |
UniProt ID | P42568 |
NCBI | NP_004520.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | myeloid/lymphoid or mixed-lineage leukemia (tritho Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human AF9 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AF9 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human AF9 by WB and IP.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-AF9 antibody used for WB at 0.1 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate.