Rabbit AAGAB Antibody

SKU: orb1520791

Description

AAGAB Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:10,000 - 1:25,000; IP - 50 µl/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 265 and C-terminus
• Target: AAGAB
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

alpha- and gamma-adaptin-binding protein p34; KPPP1; p34; PPKP1; PPKP1A

Detection of human AAGAB by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, U2OS, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AAGAB antibody (orb1520791) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse AAGAB by western blot. Samples: Whole cell lysate (10 µg) from BeWo, AlphaTC1 Clone 9, and A20 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AAGAB antibody (orb1520791) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human AAGAB by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AAGAB antibody (orb1520791) used for IP at 1 µg per reaction.

Quick Database Links

Gene Symbol

AAGAB

UniProt

Q6PD74

RefSeq (Protein):NP_078942.3