Rabbit 6-His Tag Antibody (HRP)

SKU: orb1530866

Description

6-His Tag Antibody

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Dilution Range: WB - 1:1,000 - 1:30,000; IHC - Not applicable; ICC - 1:200 - 1:500; ELISA - 1:10,000 - 1:100,000
• Application Notes:
  Format: Whole IgG. Application Information: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Target: 6-His Tag
• Purification: Antigen Affinity Purified
• Conjugation: HRP

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) containing 0.2% rAlbumin and 0.05% Pro-Clean 400
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection of 6-His-tagged protein by WB.Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-6-His antibody orb1530866 at 0.04 µg/ml (1:25, 000).

Quick Database Links

Gene Symbol

6-His Tag