Rabbit 14-3-3 theta Antibody

SKU: orb1525380

Description

14-3-3 theta Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 125 and 175
• Target: 14-3-3 theta
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

14-3-3; 14-3-3 protein tau; 14-3-3 protein T-cell; 14-3-3 protein theta; 14-3-3 theta; 1C5; HS1; Protein HS1; protein, theta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

Detection of human 14-3-3-theta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-14-3-3-theta antibody orb1525380 used for IP at 6 µg/mg lysate.

Detection of human 14-3-3-theta by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-14-3-3-theta (Cat. No. orb1525380) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

14-3-3 theta

UniProt

P27348

RefSeq (Protein):NP_006817.1