RAB7A Antibody

• Catalog Number: orb1260751
• Category: Antibodies
• Description: RAB7A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RAB7A (NP_004628.4).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 23kDa
• Target: RAB7A
• UniProt ID: P51149
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: RAB7A, CMT2B, PRO2706, RAB7, Ras-associated protei
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using RAB7A antibody (orb1260751) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunofluorescence analysis of C6 cells using RAB7A antibody (orb1260751) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using RAB7A antibody (orb1260751) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS cells using RAB7A antibody (orb1260751) at dilution of 1:100. Blue: DAPI for nuclear staining.