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Item 1 of 3
RAB5A Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1727965
| Catalog Number | orb1727965 |
|---|---|
| Category | Antibodies |
| Description | RAB5A Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Rab5 |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 22 kDa |
| Target | RAB5A |
| UniProt ID | P20339 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | RAB5; RAB 5; RAB 5A; RAB5A member RAS oncogene fam Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Rab5 antibody (orb1727965) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1727965) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue with Rabbit anti-Rab5 antibody (orb1727965) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1727965) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Rab5 on different lysates with Rabbit anti-Rab5 antibody (orb1727965) at 1/1000 dilution. Lane 1: MCF-7 cell lysate, Lane 2: Hela cell lysate, Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa, Observed band size: 24 kDa, Exposure time: 1 minute, 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (orb1727965) at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300000 dilution was used for 1 hour at room temperature.
