Rab5a Antibody

SKU: orb333921

Description

Mouse monoclonal antibody to Rab5a

Images & Validation

• Tested Applications: FC, ICC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 3H5C11
• Immunogen: Purified recombinant fragment of human Rab5a (AA: 1-215) expressed in E. Coli.
• Target: Rab5a
• Molecular Weight: 23.7kDa

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

RAB5

Immunofluorescence analysis of Hela cells using Rab5a antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded colon cancer tissues using Rab5a antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded duodenum tissues using Rab5a antibody

Western blot analysis of human recombinant protein using Rab5a antibody

Western blot analysis of HEK293 using Rab5a antibody

Western blot analysis of Hela (Lane1) and K562 (Lane2) cell lysate using Rab5a antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of Hela cells using Rab5a antibody

Quick Database Links

Gene Symbol

Rab5a

UniProt

P20339

NCBI Gene ID

5868