RAB3D Antibody (FITC)

SKU: orb751915

Description

Mouse monoclonal antibody against RAB3D conjugated to FITC

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution was sufficient for detection of RAB3D in 10 µg of A549, T-47D cells, mouse and rat brain, kidney by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5000000000
• Immunogen: Full length recombinant RAB3D protein
• Target: RAB3D
• Molecular Weight: 24 kDa
• Purification: Protein G Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Member RAS Oncogene Family, D2-2, GOV, RAB16, RAD3D, Ras-Related Protein Rab-3D

Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and RAB3D showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9. Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing RAB3D. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody.

Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9. Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney. Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Quick Database Links

Gene Symbol

RAB3D

UniProt

O95716

NCBI Gene ID

9545

RefSeq (Protein):NP_004274