RAB3D Antibody: Biotin

• Catalog Number: orb751914
• Category: Antibodies
• Description: Mouse monoclonal antibody against RAB3D conjugated to Biotin
• Target: RAB3D
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Biotin
• Reactivity: Human, Mouse, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Purification: Protein G Purified
• Immunogen: Full length recombinant RAB3D protein
• UniProt ID: O95716
• MW: 24 kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000)
• Application notes: A 1:1000 dilution of SMC-614 was sufficient for detection of RAB3D in 10 µg of A549, T-47D cells, mouse and rat brain, kidney by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
• Clone Number: 5000000000
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Member RAS Oncogene Family antibody, D2-2 antibody
• Research Area: Cancer Biology, Cell Biology, Neuroscience, Protei
• Note: For research use only
• Entrez: 9545
• NCBI: NP_004274

Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and RAB3D showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9. Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing RAB3D. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody.

Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9. Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney. Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.