RAB35 Antibody (C-term)

SKU: orb1788095

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:25
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This RAB35 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 166-199 amino acids from the C-terminal region of human RAB35. Antigen Region: 166-199 aa.
• Target: RAB35 (HGNC:9774)
• Molecular Weight: 23025 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAB35, RAB1C, RAY

Immunohistochemical analysis of paraffin-embedded H.small intestine section using RAB35 Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

All lanes: Anti-RAB35 Antibody (C-term) at 1:1000 dilution. Lane 1: human brain lysates. Lane 2: Hela whole cell lysates. Lane 3: HepG2 whole cell lysates. Lane 4: mouse brain lysates. Lane 5: NIH/3T3 whole cell lysates. Lane 6: THP-1 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

RAB35 (HGNC:9774)

UniProt

Q15286