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RAB27A Antibody
SKU: orb1274470
Featured
Description
Research Area
Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1). |
| Target | RAB27A |
| Molecular Weight | Observed: 25kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27
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Western blot analysis of extracts of U-937 cells, using RAB27A antibody (orb1274470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Western blot analysis of extracts from normal (control) and RAB27A knockout (KO) HeLa cells, using RAB27A antibody (orb1274470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human adenomyosis using RAB27A Antibody (orb1274470) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using RAB27A Antibody (orb1274470) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF7 cells using RAB27A antibody (orb1274470). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
RAB27A
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
RAB27A Antibody (orb1274470)
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