RAB23 Antibody

SKU: orb1939286

Description

RAB23 Antibody

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution range: IF - 1:25, FC - 1:25, WB - 1:2000
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Molecular Weight: 26659 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Disclaimer: For research use only

All lanes: Anti-RAB23 Antibody at 1:2000 dilution. Lane 1: human brain lysate. Lane 2: mouse brain lysate. Lane 3: MDA-MB-231 whole cell lysate. Lane 4: mouse Cerebellum lysate. Lane 5: human Cerebellum lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling RAB23 with AM2026a at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG (174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Overlay histogram showing U-2 OS cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

RAB23 Antibody western blot analysis in mouse brain tissue lysates (35 μg/lane). This demonstrates the RAB23 antibody detected the RAB23 protein (arrow).

Quick Database Links

UniProt

Q9ULC3