You have no items in your shopping cart.
Cart summary
Item 1 of 1
QKI (pan) Antibody: Biotin
Catalog Number: orb150957
| Catalog Number | orb150957 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to QKI (Biotin). QKI is also called Protein Quaking or HqkI. QKI is an RNA-binding protein that plays a central role in myelinization. QKI acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation, and is itself, regulated by alternative splicing. QKI is expressed in the frontal cortex of brain, but is shown to be down-regulated in the brain of schizophrenic patients.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | N147/6 (Formerly sold as S147-6) |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG2b |
| Immunogen | Fusion protein amino acids 1-341 (full-length) of human QKI-5. Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical) > 90% identity with QKI-6, QKI-7 and QKI-7b. |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000) |
| Conjugation | Biotin |
| MW | 36-38kDa |
| Target | QKI |
| Entrez | 9444 |
| UniProt ID | Q96PU8 |
| NCBI | NP_006766.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
| Alternative names | Protein quaking antibody, Hqk antibody, HqkI antib Read more... |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-467 was sufficient for detection of Pan-QKI in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-QKI (pan) Monoclonal Antibody, Clone N147/6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-QKI (pan) Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) QKI (pan) Antibody (D) Composite.
