Pyruvate Kinase (PKM2) Antibody (N-term)

SKU: orb1939500

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB5870
• Immunogen: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-151 amino acids from the N-terminal region of human Pyruvate Kinase (PKM2). Antigen Region: 121-151 aa.
• Target: PKM
• Molecular Weight: 57937 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP3, PK2, PK3, PKM2

All lanes: Anti-PKM2 (K136) antibody at 1:2000 dilution. Lane 1: MCF-7 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: NIH/3T3 whole cell lysates. Lane 4: PC-12 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PKM2 (K136) antibody at 1:2000 dilution. Lane 1: MCF-7 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: THP-1 whole cell lysates. Lane 4: NIH/3T3 whole cell lysates. Lane 5: PC-12 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PKM

UniProt

P14618

RefSeq (Protein):NP_001193726.1, NP_002645.3, NP_872271.1, NP_001193728.1, NP_001193725.1, NP_872270.1, NP_001193727.1