PYGB Antibody

• Catalog Number: orb1257037
• Category: Antibodies
• Description: PYGB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:20 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 105kDa
• Target: PYGB
• UniProt ID: P11216
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PYGB, GPBB, Phosphorylase, glycogen, Phosphorylase
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:20 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PYGB antibody (orb1257037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of MCF-7 cells using PYGB antibody (orb1257037). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HepG2 cells, using 3 ug PYGB antibody (orb1257037). Western blot was performed from the immunoprecipitate using PYGB antibody (orb1257037) at a dilution of 1:1000.