PYCR1 Antibody

SKU: orb1260464

Description

PYCR1 Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2).
• Target: PYCR1
• Molecular Weight: Observed: 35kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Pyrroline-5-carboxylate reductase 1, mitochondrial, P5C reductase 1, P5CR 1, PYCR1

Western blot analysis of extracts of various cell lines, using PYCR1 antibody (orb1260464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat liver using PYCR1 antibody (orb1260464) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using PYCR1 antibody (orb1260464) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using PYCR1 antibody (orb1260464) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using PYCR1 antibody (orb1260464) at dilution of 1:200 (40x lens).

Quick Database Links

Gene Symbol

PYCR1

UniProt

P32322