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PXR Rabbit Polyclonal Antibody

Catalog Number: orb6823

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6823
CategoryAntibodies
DescriptionPXR Rabbit Polyclonal Antibody
TargetNR1I2
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse
Predicted ReactivityBovine, Equine, Porcine, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human PXR (51-150/434aa)
UniProt IDO75469
RRIDAB_10920090
MW48 kDa
Tested applicationsFC, WB
Dilution rangeWB=1:500-2000, Flow-Cyt=2ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesBXR; NR1I2; Nuclear receptor subfamily 1 group I m
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NoteFor research use only
PXR Rabbit Polyclonal Antibody

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PXR antibody (orb6823), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

PXR Rabbit Polyclonal Antibody

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-PXR (orb6823) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.

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