PXN Antibody (Y118)

SKU: orb1930050

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD37636
• Immunogen: This PXN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-125 amino acids from human PXN. Antigen Region: 94-125 aa.
• Target: PXN (HGNC:9718)
• Molecular Weight: 64505 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Paxillin, PXN

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PXN Antibody (Y118), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of PXN (arrow) using rabbit polyclonal PXN Antibody (Y118). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2).

All lanes: Anti-PXN Antibody (Y118) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PXN (HGNC:9718)

UniProt

P49023

RefSeq (Protein):NP_001230685.1, NP_001074324.1, NP_002850.2, NP_079433.3