PXN Antibody (Y118)

• Catalog Number: orb1930050
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PXN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-125 amino acids from human PXN.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P49023
• MW: 64505 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB15414
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Paxillin, PXN
• Research Area: Cancer Biology, Immunology & Inflammation, Signal
• Note: For research use only
• NCBI: NP_002850.2, NP_079433.3, NP_001230685.1, NP_001074324.1
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PXN Antibody (Y118), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of PXN (arrow) using rabbit polyclonal PXN Antibody (Y118). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2).

All lanes: Anti-PXN Antibody (Y118) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.