Puromycin aminonucleoside

SKU: orb1307832

Description

Puromycin aminonucleoside (NSC 3056) is a purine analog antibiotic that reversibly inhibits dipeptidyl peptidase and cytosolic alanine aminopeptidase. It is widely used to establish experimental nephrotic syndrome models in vivo, as it induces podocyte injury and apoptosis through oxidative stress-dependent mechanisms.

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research, Protein Biochemistry

Key Properties

• CAS Number: 58-60-6
• MW: 294.31
• Purity: 99.90%
• Formula: C₁₂H₁₈N₆O₃
• SMILES: CN(C)c1ncnc2n(cnc12)[C@@H]1O[C@H](CO)[C@@H](N)[C@H]1O
• Target: Antibiotic,Aminopeptidase,Antibacterial,Proteasome,Apoptosis
• Solubility: H2O:33.33 mg/mL (113.25 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.8 mM);DMSO:31 mg/mL (105.33 mM)

Bioactivity

• In Vivo:
  Rats administered Puromycin aminonucleoside (100 mg/kg, s.c.) exhibited reduced weight gain and elevated serum creatinine levels compared to controls. The podocyte count per glomerulus in control rats stood at 95.5±17.6, dropping to 90.7 by Day 4 in rats with Puromycin aminonucleoside (8 mg/100 g, i.v.)-induced nephrosis. Furthermore, nephrin levels per glomerulus in controls were 1.02±0.11 fmol, which decreased significantly in nephrosis rats to 0.46±0.06 fmol and 0.35±0.04 fmol on Days 4 and 7, respectively. This reduction in nephrin per podocyte was closely linked to proteinuria development in rats affected by Puromycin aminonucleoside nephrosis .
• In Vitro:
  Puromycin aminonucleoside -induced podocyte apoptosis is p53 dependent. Puromycin aminonucleoside causes podocyte apoptosis in a time-dependent manner. The IC50 values for PMAT-expressing and vector-transfected cells are 48.9 and 122.1 μM, respectively, suggesting expression of PMAT-enhanced cell sensitivity to Puromycin aminonucleoside. Puromycin aminonucleoside uptake in PMAT-expressing cells is fourfold higher at pH 6.6 than that at pH 7.4. Puromycin aminonucleoside (30 μg/mL) markedly enhances p53 protein levels in podocytes. Puromycin aminonucleoside (250 μM) is toxic to both PMAT-expressing and vector-transfected cells .

Storage & Handling

• Storage: keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

dipeptidyl peptidase II, Dipeptidyl Peptidase, DPP, antibiotic, Antibiotic, Aminopeptidase, apoptosis, Apoptosis, Bacterial, NSC-3056, NSC3056, Inhibitor, inhibit, NSC 3056, Puromycin aminonucleoside

Compound Identifiers

PubChem CID

6020