Punicalin

SKU: orb1220962

Description

Punicalin exerts anti-inflammatory antioxidative and anti-hepatotoxic activities it shows inhibitory activity on HIV-1 reverse transcriptase in a dose-dependent manner with an IC50 of 0.11 microg/ml (0.14 microM).

Key Properties

• CAS Number: 65995-64-4
• MW: 782.52
• Purity: >98% (HPLC)
• Formula: C₃₄H₂₂O₂₂
• SMILES: OC1O[C@@H]2COC(=O)c3cc(O)c(O)c(O)c3-c3c(O)c(O)c4oc(=O)c5c(c(O)c(O)c6oc(=O)c3c4c56)-c3c(O)c(O)c(O)cc3C(=O)O[C@H]2[C@H](O)[C@H]1O
• Target: HIV
• Solubility: In Vitro: H2O : 50 mg/mL (63.90 mM)

Bioactivity

• In Vivo:
  Animal model: Carrageenan (HY-125474)-induced Edema in rats. Dosage: 5 mg/kg. Administration: s. c. Result: Showed significant anti-inflammatory effect against carrageenan induced paw edema at all time intervals, but decreased the effects. with larger dose. Animal model: LPS (HY-D1056)-induced acute lung injury mice. Dosage: 10 mg/kg. Administration: i.p. Result: Reduced the mortality and attenuated the severity of lung pathological injury. Ameliorated LPS (HY-D1056)-induced lung edema and vascular leakage.
• In Vitro:
  Western blot analysis. Cell line: J774A. Concentration: 0, 25, 50, 100 μM. Incubation time: The cells were pretreated with punicalin for one hour, followed by treated with Lipopolysaccharide (HY-D1056) for 5.5 h, ATP for half an hour, respectively. Result: Reduced the increased protein expression of NLRP3, ASC, Caspase-1 and GSDMD-N. Cell Cycle Analysis Cell line: SH-SY5Y. Concentration: 10, 20 μM. Incubation time: 24-72 h. Result: Alleviated the rising of the G0/G1 phase ratio. Ameliorated OGD/R-mediated expression of cyclin B1, CDK1, p21 and phosphorylation of CDK1 at 20 μM. Cell Viability Assay Cell line: J774A. Concentration: 0, 25, 50, 100 μM. Incubation time: 24 h. Result: Promoted cell viability and decreased the release of pro-inflammatory cytokines.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

HIV