PU.1 Antibody / SPI1

• Catalog Number: orb1151758
• Category: Antibodies
• Description: Mouse monoclonal antibody to PU.1
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 16-170 was used as the immunogen for the recombinant PU.1 antibody.
• UniProt ID: P17947
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant PU.1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rPU1/2146
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lymph node with recombinant PU.1 antibody (clone rPU1/2146). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with recombinant PU.1 antibody (clone rPU1/2146). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant PU.1 antibody (clone rPU1/2146) as confirmation of integrity and purity.