PTPRC Antibody

• Catalog Number: orb1258278
• Category: Antibodies
• Description: PTPRC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 890-990 of human PTPRC (NP_002829.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 185kDa
• Target: PTPRC
• UniProt ID: P08575
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Receptor-type tyrosine-protein phosphatase C, Leuk
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse thymus, using PTPRC antibody (orb1258278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse lung using PTPRC antibody (orb1258278) (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using PTPRC antibody (orb1258278) (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using PTPRC antibody (orb1258278) (40x lens).

Immunofluorescence analysis of Jurkat cells using PTPRC antibody (orb1258278) at dilution of 1:100. Blue: DAPI for nuclear staining.