PTPRC Antibody (N-term)

• Catalog Number: orb1938003
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB20781
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 147486 Da
• Target: This PTPRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids of human PTPRC.
• UniProt ID: P08575
• NCBI: NP_002829.3, NP_563578.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Receptor-type tyrosine-protein phosphatase C, Leuk
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PTPRC Antibody (N-term) at 1:2000 dilution. Lane 1:Ramos cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 180kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PTPRC Antibody (N-term) at 1:1000 dilution + Jurkat whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 147 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PTPRC Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.