PTPN6 Antibody (Center)

• Catalog Number: orb1931433
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PTPN6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-277 amino acids from the Central region of human PTPN6.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P29350
• MW: 67561 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:100, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Clone Number: RB17225
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tyrosine-protein phosphatase non-receptor type 6,
• Note: For research use only
• NCBI: NP_002822.2, NP_536858.1, NP_536859.1

Immunofluorescent analysis of U251 cells, using PTPN6 Antibody (Center). Diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of PTPN6 Antibody (Center) in HL-60 cell line lysates (35 ug/lane).PTPN6 (arrow) was detected using the purified Pab.

Western blot analysis of PTPN6 (arrow) using rabbit polyclonal PTPN6 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2). (2 ug/ml)