PTPN6 Antibody (Center)

SKU: orb1931433

Description

PTPN6 Antibody (Center)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: IF - 1:100, WB - 1:1000, IHC-P - 1:100-500
• Reactivity: Human, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 67561 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

HCP, PTP1C

Immunofluorescent analysis of U251 cells, using PTPN6 Antibody (Center). Diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of PTPN6 Antibody (Center) in HL-60 cell line lysates (35 ug/lane).PTPN6 (arrow) was detected using the purified Pab.

Western blot analysis of PTPN6 (arrow) using rabbit polyclonal PTPN6 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2). (2 ug/ml)

Quick Database Links

UniProt

P29350