PTPN22 Antibody

• Catalog Number: orb1260113
• Category: Antibodies
• Description: PTPN22 Antibody
• Target: PTPN22
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PTPN22 (NP_036543.4).
• UniProt ID: Q9Y2R2
• MW: Observed: 107kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Tyrosine-protein phosphatase non-receptor type 22,
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only

Western blot analysis of extracts of various cell lines, using PTPN22 antibody (orb1260113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunofluorescence analysis of C6 cells using PTPN22 antibody (orb1260113) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L-929 cells using PTPN22 antibody (orb1260113) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using PTPN22 antibody (orb1260113) at dilution of 1:100. Blue: DAPI for nuclear staining.