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Item 1 of 5
Item 1 of 5
PTP4A2 Antibody (Center)
Catalog Number: orb1929772
| Catalog Number | orb1929772 |
|---|---|
| Category | Antibodies |
| Description | affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB18592 |
| Tested applications | FC, IF, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 19127 Da |
| Target | This PTP4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-59 amino acids from the Central region of human PTP4A2. |
| UniProt ID | Q12974 |
| NCBI | NP_001182029.1, NP_001182030.1, NP_536316.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Protein tyrosine phosphatase type IVA 2, HU-PP-1, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of widr cells using PTP4A2 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence analysis of PTP4A2 Antibody (Center) with paraffin-embedded human prostate carcinoma tissue.0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Formalin-fixed and paraffin-embedded human prostate carcinoma with PTP4A2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PTP4A2 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the PTP4A2 antibody detected the PTP4A2 protein (arrow).
Anti-PTP4A2 Antibody (Center) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
