PTP4A2 Antibody (Center)

• Catalog Number: orb1929772
• Category: Antibodies
• Description: PTP4A2 Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q12974
• MW: 19127 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PRL2, PTPCAAX2
• Research Area: Cancer Biology, Cell Biology, Signal Transduction
• Note: For research use only

Flow cytometric analysis of widr cells using PTP4A2 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence analysis of PTP4A2 Antibody (Center) with paraffin-embedded human prostate carcinoma tissue.0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Formalin-fixed and paraffin-embedded human prostate carcinoma with PTP4A2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PTP4A2 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the PTP4A2 antibody detected the PTP4A2 protein (arrow).

Anti-PTP4A2 Antibody (Center) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.