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Item 1 of 5
PTK2 Antibody (Center)
Catalog Number: orb1928396
| Catalog Number | orb1928396 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB20775 |
| Tested applications | FC, IF, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 119233 Da |
| Target | This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the Central region of human PTK2. |
| UniProt ID | Q05397 |
| NCBI | NP_722560.1, NP_001186578.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Focal adhesion kinase 1, FADK 1, Focal adhesion ki Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of PTK2 Antibody (Center) in T47D cell line lysates (35 ug/lane). PTK2 (arrow) was detected using the purified Pab.
Flow cytometric analysis of K562 cells using PTK2 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PTK2 (arrow) using rabbit polyclonal PTK2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK2 gene (Lane 2).
Confocal immunofluorescent analysis of PTK2 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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