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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | PTEN (Phospho-Ser380/Thr382/Thr383) antibody was raised against a peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S (p) -D-T (p) -T (p) -D-S) derived from Human PTEN. |
| Target | PTEN |
| Molecular Weight | 54 kDa |
| Purification | Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, Mutated in multiple advanced cancers 1, Protein-tyrosine phosphatase PTEN
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Western blot analysis of lysed extracts from HeLa cells using PTEN (Phospho-Ser380/Thr382/Thr383).
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN (Phospho-Ser380/Thr382/Thr383). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PTEN (Phospho-Ser380/Thr382/Thr383).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000305.3 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
PTEN Antibody (orb1253808)
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