PTEN Antibody (N-term)

SKU: orb39110

Description

Purified rabbit polyclonal antibody.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10-50, IF/ICC: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB05082
• Immunogen: Synthetic Peptide
• Target: PTEN
• Molecular Weight: 47166
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-"Phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase PTEN antibody, anti-Mutated in multiple advanced cancers 1 antibody, anti-MMAC1 antibody, anti-TEP2" antibody

PTEN Antibody (N-term) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the PTEN antibody detected the PTEN protein (arrow).

Western blot analysis of PTEN (arrow) using rabbit PTEN Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTEN gene (Lane 2).

Confocal immunofluorescent analysis of PTEN Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTEN antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

PTEN

UniProt

P60484

RefSeq (Protein):NP_000305.3

Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)

PubMed: 32050796