PTEN Antibody (C-term F279)

SKU: orb1928502

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This PTEN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-295 amino acids from the C-terminal region of human PTEN. Antigen Region: 264-295 aa.
• Target: PTEN
• Molecular Weight: 47166 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phosphatidylinositol 3,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1

The anti-PTEN Pab is used in Western blot to detect PTEN in HL-60 cell lysate.

Confocal immunofluorescent analysis of PTEN Antibody (C-term F279) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

PTEN Antibody (C-term F279) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTEN Antibody (C-term F279) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

PTEN

UniProt

P60484

RefSeq (Protein):NP_000305.3