PTCH Antibody

• Catalog Number: orb1247057
• Category: Antibodies
• Description: PTCH Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: ELQDVECEERPR
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PTCH1
• UniProt ID: Q13635
• NCBI: NP_000255.1, NP_001077075.1, NP_001077073.1, NP_001077072.1, NP_001077071.1, NP_001077074.1, NP_001077076.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PTCH1, PTCH, patched homolog (Drosophila), HGNC:95
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Immunohistochemistry:In paraffin embedded Human Colon shows textured nuclear staining of the absorptive cells. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml. This produt has been successfully used in IHC on Human: Abiria SA et al, J Neurooncol. 2014 May 28. PMID: 24867209.Immunofluorescence: Expression of the protein seen in the cytoplasm of HeLa and in the Golgi/membranes of NIH3T3 cells. Recommended concentration: 5ug/ml. This produt has been successfully used in IF on Human: Abiria SA et al, J Neurooncol. 2014 May 28. PMID: 24867209.
• Expiration Date: 12 months from date of receipt.

orb1247057 (3.75 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247057 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1247057 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi/membrane staining.