PSMG1 Antibody

SKU: orb1249550

Description

PSMG1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and U2OS cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KLMTTNEIQSNIYT
• Target: PSMG1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

PSMG1, proteasome (prosome, macropain) assembly chaperone 1, C21LRP, DSCR2, LRPC21, PAC1, Down syndrome critical region gene 2, Down syndrome critical region protein 2, chromosome 21 leucine-rich protein, leucine rich protein C21-LRP, proteasome assembling chaperone 1

orb1249550 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic and Golgi apparatus staining.

orb1249550 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing Golgi apparatus and some cytoplasmic staining.

orb1249550 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

PSMG1

UniProt

O95456

RefSeq (Protein):NP_003711.1, NP_001248753.1, NP_001307724.1, NP_982257.1