PSMB9 Antibody (C-term)

• Catalog Number: orb1928436
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22334
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 23264 Da
• Target: This PSMB9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-219 amino acids from the C-terminal region of human PSMB9.
• UniProt ID: P28065
• NCBI: NP_002791.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Proteasome subunit beta type-9, Low molecular mass
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PSMB9 Antibody (C-term) in HL-60 cell line lysates (35 ug/lane). PSMB9 (arrow) was detected using the purified Pab.

PSMB9 Antibody (C-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PSMB9 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-PSMB9 Antibody (C-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.