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PSMB8 Antibody
SKU: orb1256454
Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 163-272 of human PSMB8 (NP_004150.1). |
| Target | PSMB8 |
| Molecular Weight | Observed: 23-28kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PSMB8, proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7), D6S216, D6S216E, LMP7, MGC1491, PSMB5i, RING10, beta5i, OTTHUMP00000029420, OTTHUMP00000029421, OTTHUMP00000062981, low molecular weight protein 7, macropain subunit C13, multicatalytic endopeptidase complex subunit C13, protease component C13, macropain) subunit beta type 8 (large multifunctional protease 7), proteasome beta 8 subunit, proteasome catalytic subunit 3i, proteasome subunit Y2, proteasome subunit beta 5i, proteasome-related gene 7, JMP, ALDD, NKJO
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Western blot analysis of extracts of various cell lines, using PSMB8 antibody (orb1256454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using PSMB8 antibody (orb1256454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using PSMB8 antibody (orb1256454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using PSMB8 antibody (orb1256454) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PSMB8 antibody (orb1256454). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
PSMB8
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
PSMB8 Antibody (orb1256454)
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