You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1258358 |
---|---|
Category | Antibodies |
Description | PSMB5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human PSMB5 (NP_002788.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 21kDa |
Target | PSMB5 |
UniProt ID | P28074 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Proteasome subunit beta type-5, Macropain epsilon Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using PSMB5 antibody (orb1258358) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using PSMB5 antibody (orb1258358) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using PSMB5 antibody (orb1258358). Blue: DAPI for nuclear staining.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IP, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
Filter by Rating