PSMA3 Antibody

• Catalog Number: orb1260915
• Category: Antibodies
• Description: PSMA3 Antibody
• Target: PSMA3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human PSMA3 (NP_002779.1).
• UniProt ID: P25788
• MW: Observed: 28kDa
• Tested applications: IF, IP, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HC8, PSC3, proteasome subunit alpha type-3, macrop
• Research Area: Cell Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PSMA3 antibody (orb1260915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of MCF-7 cells using PSMA3 antibody (orb1260915). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HL-60 cells, using 3 ug PSMA3 antibody (orb1260915). Western blot was performed from the immunoprecipitate using PSMA3 antibody (orb1260915) at a dilution of 1:1000.