PSMA3 Antibody

• Catalog Number: orb1260915
• Category: Antibodies
• Description: PSMA3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human PSMA3 (NP_002779.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 28kDa
• Target: PSMA3
• UniProt ID: P25788
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HC8, PSC3, proteasome subunit alpha type-3, macrop
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PSMA3 antibody (orb1260915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of MCF-7 cells using PSMA3 antibody (orb1260915). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HL-60 cells, using 3 ug PSMA3 antibody (orb1260915). Western blot was performed from the immunoprecipitate using PSMA3 antibody (orb1260915) at a dilution of 1:1000.