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PSD95 Antibody
SKU: orb1822496
Featured
Description
Research Area
Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | AM, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB (1:250), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Reactivity | Bovine, Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | 6G6 |
| Immunogen | Recombinant rat PSD-95 |
| Target | PSD95 |
| Molecular Weight | 75kDa |
| Purification | Protein A Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PSD 95, PSD-95, PSD95, DLG4, SAP90, Synapse-associated protein 90, Postsynaptic density protein 95, Disks large homolog 4
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Junction staining.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X.
Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_062567.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
PSD95 Antibody (orb1822496)
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