PSD95 Antibody

• Catalog Number: orb1822496
• Category: Antibodies
• Description: Mouse Anti-Rat PSD95 Monoclonal IgG2a
• Target: PSD95
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Protein A Purified
• Immunogen: Recombinant rat PSD-95
• UniProt ID: P31016
• MW: 75kDa
• Tested applications: AM, ICC, IF, IHC, WB
• Dilution range: WB (1:250), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Application notes: 1 µg/ml was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.
• Clone Number: 6G6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PSD 95 Antibody, PSD-95 Antibody, DLG4 Antibody, S
• Note: For research use only
• Entrez: 29495
• NCBI: NP_062567.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Junction staining.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X.

Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.