PSCA Antibody (Center)

• Catalog Number: orb1936039
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB11935
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, WB: 1:2000, WB: 1:1000-1:2000, WB: 1:1000-1:2000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 11959 Da
• Target: This PSCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the Central region of human PSCA.
• UniProt ID: O43653
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Prostate stem cell antigen, PSCA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PSCA Antibody (Center) at 1:1000 dilution. Lane 1: mouse stomach lysate. Lane 2: mouse kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PSCA Antibody (Center) at 1:2000 dilution. Lane 1: Mouse kidney lysate. Lane 2: Mouse stomach lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PSCA Antibody (Center) at 1:2000 dilution. Lane 1: Mouse kidney lysate. Lane 2: Mouse stomach lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PSCA Antibody (Center) at 1:1000-1:2000 dilution. Lane 1: Mouse kidney lysate. Lane 2: Mouse stomach lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PSCA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PSCA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-PSCA Antibody (Center) at 1:1000-1:2000 dilution. Lane 1: Mouse kidney lysate. Lane 2: Mouse stomach lysate. Lane 3: Mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.