PSCA Antibody (Center)

SKU: orb1936039

Description

PSCA Antibody (Center)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000-1:2000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 11959 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-PSCA Antibody (Center) at 1:1000 dilution. Lane 1: mouse stomach lysate. Lane 2: mouse kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PSCA Antibody (Center) at 1:2000 dilution. Lane 1: Mouse kidney lysate. Lane 2: Mouse stomach lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PSCA Antibody (Center) at 1:2000 dilution. Lane 1: Mouse kidney lysate. Lane 2: Mouse stomach lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PSCA Antibody (Center) at 1:1000-1:2000 dilution. Lane 1: Mouse kidney lysate. Lane 2: Mouse stomach lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PSCA Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PSCA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-PSCA Antibody (Center) at 1:1000-1:2000 dilution. Lane 1: Mouse kidney lysate. Lane 2: Mouse stomach lysate. Lane 3: Mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

O43653