PSA Antibody (Center)

SKU: orb1929985

Description

PSA Antibody (Center)

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 103276 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PSA

PSA Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

PSA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of PSA Antibody (Center) in MCF-7 and Hela cell line lysates (35 ug/lane). PSA (arrow) was detected using the purified Pab.

All lanes: Anti-PSA Antibody (Center) at 1:2000 dilution. Lane 1: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size: 110kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PSA Antibody (Center) at 1:2000 dilution. Lane 1: mouse brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size: 110kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P55786