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Item 1 of 5
PSA Antibody (Center)
Catalog Number: orb1929985
| Catalog Number | orb1929985 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB19101 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 103276 Da |
| Target | This PSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 536-565 amino acids from the Central region of human PSA. |
| UniProt ID | P55786 |
| NCBI | NP_006301.3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Puromycin-sensitive aminopeptidase, PSA, Cytosol a Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
PSA Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PSA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of PSA Antibody (Center) in MCF-7 and Hela cell line lysates (35 ug/lane). PSA (arrow) was detected using the purified Pab.
All lanes: Anti-PSA Antibody (Center) at 1:2000 dilution. Lane 1: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size: 110kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-PSA Antibody (Center) at 1:2000 dilution. Lane 1: mouse brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size: 110kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
