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PRTN3/Myeloblastin Antibody
Catalog Number: orb1535175
| Catalog Number | orb1535175 |
|---|---|
| Category | Antibodies |
| Description | PRTN3/Myeloblastin Antibody |
| Target | PRTN3 / Myeloblastin |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Concentration | 0.5 mg/ml |
| Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | Peptide with sequence C-HNVRTQEPTQQ, from the internal region of the protein sequence according to NP_002768.3. |
| Tested applications | ELISA, IHC, IHC-P, WB |
| Dilution range | IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:8000), WB (0.3 - 1 µg/ml) |
| Application notes | Further information: Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 27kD band observed in Human Spleen lysates (calculated MW of 27.8kD according to NP_002768.3). Recommended concentration: 1-3 ug/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | PRTN3, ACPA, Azurophil granule protein 7, C-ANCA a Read more... |
| Note | For research use only |
E12324 (0.3 µg/ml) staining of Human Spleen lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
