PRSS3 Antibody (Center)

• Catalog Number: orb1937860
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB31598
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 32529 Da
• Target: This PRSS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-163 amino acids from the Central region of human PRSS3.
• UniProt ID: P35030
• NCBI: NP_002762.2, NP_031369.2, NP_001184026.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Trypsin-3, Brain trypsinogen, Mesotrypsinogen, Ser
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PRSS3 Antibody (Center) at 1:1000 dilution. Lane 1: MDA-MB-231 whole cell lysate. Lane 2: human pancreas lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PRSS3 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

PRSS3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PRSS3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.