PRS4 Antibody (C-term)

SKU: orb1788378

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:25, IF: 1:25, WB: 1:1000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This PRS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of human PRS4. Antigen Region: 408-436 aa.
• Target: PSMC1
• Molecular Weight: 49185 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

26S protease regulatory subunit 4;26S protease regulatory subunit 4; 26S protease regulatory subunit 4; 26S proteasome AAA-ATPase subunit RPT2; 26S protease regulatory subunit 4; Proteasome 26S subunit ATPase 1

Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. colon section using PRS4 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from A549, Hela, Jurkat, mouse NIH/3T3 cell line (from left to right), using PRS4 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Western blot analysis of lysates from A549, Hela cell line, mouse lung, rat lung tissue (from left to right), using PRS4 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Fluorescent image of Hela cells stained with PRS4 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Fluorescent image of Hela cells stained with PRS4 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Quick Database Links

Gene Symbol

PSMC1

UniProt

P62191

RefSeq (Protein):NP_002793.2