PRRX1 Antibody

SKU: orb1247066

Description

PRRX1 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 28kDa band observed in Human Heart lysates (calculated MW of 27.3kDa according to NP_073207.1). Recommended concentration: 1-3µg/mL.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GSDTPQQDNDQ
• Target: PRRX1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRRX1, paired related homeobox 1, PHOX1, PMX1, PRX1, homeobox protein PHOX1, paired mesoderm homeo box 1, paired mesoderm homeobox 1

orb1247066 (1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247066 (5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

PRRX1

UniProt

P54821

RefSeq (Protein):NP_008833.1, NP_073207.1